Study design is critical in this field, as the coordination of in-vitro cell isolation, activation, and labeling are in play, along with with in-vivo injection and imaging. Plenty of moving parts to manage. At the core of design considerations are the varios phases of in-vivo tracking – early events in the first 1-24 hours, or long term (days to weeks). Early events are often best captured by radiolabeling cells, often with Indium – this technology is biocompatible, non-immunogenic, FDA-approved and widely adaptable to all animals and translation clinical research. A rare solution for this difficult science.
Fluorescence cell labeling is an option in mouse models. For long-term tracking (more than 8 days) typically requires the transfection of cells with a reporter gene. Often immune therapy studies include hematology analysis, flow cytometry, clinical chemistry and arms for immunohistology. We know this sounds like a lot of options – contact us and we can help design something that meets your specific needs.